The question of possibility is most often a question of effort. By and large, you can use JavaScript to start an audio playing whenever you like. This "whenever" can be an IAT trial.
So, it will be possible, but it won't work "out of the box", but require a bit additional effort. And you shall plan some additional testing, for example to check if there are any latency issues if, for example, someone participates with a poor Internet connection. Preloading the audio files will be necessary to avoid issues there.